Coding

Part:BBa_K4739008

Designed by: Huaiyuan Ma   Group: iGEM23_SUSTech-Shenzhen   (2023-10-10)


VgrG3-Tenecteplase fusion protein

This part is a fusion protein of VgrG3 from V. cholerae, Tenecteplase, and 3 v5 tag for western blot. VgrG3 proteins puncture the target cell membrane to deliver the toxic effector molecules. Various VgrG proteins might be associated with different effectors and might target different cells, helping the bacteria to deal with various competitive environments and hosts. In T6SS formation of V. cholerae, VgrG3 will form a trimer, assemble to the tip of T6SS and puncture target cell.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2547
    Illegal EcoRI site found at 3019
    Illegal XbaI site found at 3517
    Illegal PstI site found at 3136
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2547
    Illegal EcoRI site found at 3019
    Illegal PstI site found at 3136
    Illegal NotI site found at 2818
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2547
    Illegal EcoRI site found at 3019
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2547
    Illegal EcoRI site found at 3019
    Illegal XbaI site found at 3517
    Illegal PstI site found at 3136
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2547
    Illegal EcoRI site found at 3019
    Illegal XbaI site found at 3517
    Illegal PstI site found at 3136
    Illegal NgoMIV site found at 3574
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None